Effect of Garlic Extracts on Monosodium Glutamate (MSG) Induced Fibroid in Wistar Rats
G.O. Obochi, S.P. Malu, M. Obi-Abang, Y. Alozie and M.A. Iyam
Effect of garlic extracts on MSG induced fibroid in wistar rats was studied. Fifteen rats were randomly assigned into 3 study groups. The animals in Group 1 (the control) received a placebo of 5.0 mL distilled water via gastric intubation. The animals in the Groups 2 and 3 were treated with 100 mg MSG/kg, or a combination of 100 mg MSG/kg + 100 mg garlic/kg, respectively in a total volume of 5.0 mL vehicle. However, the animals in Group 3 were treated with MSG only for 30 days before the commencement of treatment with garlic extracts. The fibroid was confirmed by myometry. The experiment lasted for 60 days. One day after the final exposure, the animals were euthanized by inhalation of overdose of chloroform. Blood was collected by cardiac puncture into EDTA sterilized sample bottles. Serum was prepared by centrifugation (6000× g, 30 min) and used for the analysis of serum total protein, estradiol (estrogen and serum total cholesterol. The results showed that Monosodium Glutamate (MSG) alone increased total protein, cholesterol and estradiol (estrogen), which in turn, induced fibroid in the rats. However, treatment with garlic extracts near-completely abrogated/mitigated any effects that have been induced by the MSG alone. It appears that Garlic extracts acted to remove catabolic wastes from the pelvic cavity and from uterine and ovarian tissue, thereby accelerating metabolism and lymph drainage; promoted the sloughing-off tissues; corrected imbalances of estrogen metabolism associated with excessive catechol estrogens and elevated inflammatory prostaglandins. It also appears that garlic extracts stimulated the secretion of gonadotrophins and ovarian hormones and inhibited proliferation of cancer cells at the levels of the pituitary gland; promoted the exit of cells from the golgi phase of the cycle; promoted the unliganded estrogen receptors ability to transducer growth signals from other pathways, leading to apoptosis of fibroid or tumour cells. The results of this study may offer the possibility of treating women with fibroids for extended periods of time without the need for surgery or hormone add-back.