Flock-Level Seroprevalence against Avian Pneumovirus amongst Uruguayan Broiler Chickens

G. Giossa, K. Suzuki, M. Petruccelli, G. Rodríguez, G. Trenchi and H. Trenchi

The objective of this study was to estimate the true prevalence of seropositive broiler chickens against avian pneumovirus at flock-level in Uruguay, using the Rogan-Gladen estimator in conjunction with Bayesian inference. A total of 181 pooled samples (consisting of 10 individual-chicken sera each) from the study area were examined with the enzyme-linked immunosorbent assay. All individual-chicken samples in the pools were also examined with the same assay. Forty-four pools were classified as test positive, because they included at least one individual-chicken classified as positive. The estimates for the deterministic (Rogan-Gladen approach) and stochastic (Bayesian approach) true prevalence were 30.9% [95% confidence interval (CI): 26.8-35.0%] and 31.4% (95% CI: 15.4-49.5%), respectively.